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Quantitative Biology > Subcellular Processes

arXiv:q-bio/0511013 (q-bio)
[Submitted on 12 Nov 2005]

Title:Incorporating expression data in metabolic modeling: a case study of lactate dehydrogenase

Authors:Joshua Downer, Joel R. Sevinsky, Natalie G. Ahn, Katheryn A. Resing, M. D. Betterton
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Abstract: Integrating biological information from different sources to understand cellular processes is an important problem in systems biology. We use data from mRNA expression arrays and chemical kinetics to formulate a metabolic model relevant to K562 erythroleukemia cells. MAP kinase pathway activation alters the expression of metabolic enzymes in K562 cells. Our array data show changes in expression of lactate dehydrogenase (LDH) isoforms after treatment with phorbol 12-myristate 13-acetate (PMA), which activates MAP kinase signaling. We model the change in lactate production which occurs when the MAP kinase pathway is activated, using a non-equilibrium, chemical-kinetic model of homolactic fermentation. In particular, we examine the role of LDH isoforms, which catalyze the conversion of pyruvate to lactate. Changes in the isoform ratio are not the primary determinant of the production of lactate. Rather, the total concentration of LDH controls the lactate concentration.
Comments: In press, Journal of Theoretical Biology. 27 pages, 9 figures
Subjects: Subcellular Processes (q-bio.SC); Quantitative Methods (q-bio.QM)
Cite as: arXiv:q-bio/0511013 [q-bio.SC]
  (or arXiv:q-bio/0511013v1 [q-bio.SC] for this version)
  https://doi.org/10.48550/arXiv.q-bio/0511013
Journal reference: J Theor Biol 240: 464-474 (2006)
Related DOI: https://doi.org/10.1016/j.jtbi.2005.10.007

Submission history

From: M. D. Betterton [view email]
[v1] Sat, 12 Nov 2005 20:30:13 UTC (57 KB)
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